uDISCO: A Novel Tissue Clearing Technique for Visualization of Central Nervous System
The three-dimensional nature of biological specimens poses a challenge to whole organ imaging, due to the obscuring effects of light scatter. Innovative tissue-clearing techniques, combined with advances in imaging of cleared tissue, have increasingly been under investigation as substitutes for histological methods. Researchers from Ludwig Maximilian University have established a novel tissue-clearing and whole-body imaging technique, named “ultimate DISCO” (uDISCO). Tissue clearing and shrinkage (up to 65%) were achieved by exposure to a solvent, which eliminated water and fat content over the course of several days. Using this technique,…
Read More